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15-20万个共聚焦小皿的推荐细胞接种量为15-20万个/皿，具体根据玻底直径和实验需求调整。 15mm直径玻底（面积约1.77cm²）：推荐15-20万个细胞/皿。 20mm直径玻底（面积约3.14cm²）：可按比例增加至30-40万个细胞。 用于细胞培养的培养皿和烧瓶有多种尺寸。 下面给出了不同尺寸培养容器的一些有用的细胞培养数值，例如解离溶液的表面积和体积。 35mm共聚焦皿，先加500μl的细胞悬液，过夜贴壁后再加相应质量的培养基，直接细胞混匀。 本产品的主要特点如下：1.本产品为35mm标准细胞培养皿，圆形，进口高品质聚苯乙烯 (polystyrene, PS)材质，培养皿内部表面经组织培养处理 (tissue culture treated)，适合细胞培养。
文献里面写6孔板是10万个细胞/孔，但是好像大家都不会去计数，都是凭经验。 那么4T1细胞，一皿长满了 (100mm直径的皿)能铺几个6孔板呢？ 选择合适的培养皿需要考虑的因素有：是否适合自己的细胞贴壁、培养皿的光学特性是否符合共聚焦要求。 能符合上述因素要求的培养皿，不局限于带包被的玻璃底培养皿，事实上，大家都知道塑料培养皿更便宜、相对玻璃来说，也更容易让细胞贴壁。 具体所加细胞密度依实验的目的不同灵活掌握。 注：各种单层生长的细胞在培养皿中长满的细胞数，主要取决于器皿底表面积和细胞体积的大小。 在细胞实验中，如果要进行一次激光共聚焦成像，除了要知道相关的显微镜参数设置和操作以外，样品的准备也是非常重要的一环。 由于激光共聚焦实验对观测耗材的底部有着比较高的要求，普通的培养皿，培养板或者培养瓶都不能直接用于激光共聚焦成像。
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